Owning My Own Assignment Example | Topics and Well Written Essays - 750 words

Owning My Own - Assignment Example Social responsibility in business also ensures that the company has a positive impact on its customers, stockholders, community, employees and the environment. Social responsibility helps guide a business on its mission and what it stands for and will uphold for its consumers, employees, stockholders and the community (Werther and Chandler, 2010). Discussed below are ways and procedures put in place to ensure that incorporation of self responsibility in business will satisfy consumers, employees and stockholders. Consumers are a vital part of business since they purchase the company’s products. To ensure social responsibility on consumers, the company has to strive and produce high quality and standard goods. In ensuring the quality of products, a company addresses ethical and quality standards expected in business (Werther and Chandler, 2010). A company has to produce high quality products which meet the satisfaction of the consumer. The products should not be harmful to cons umer health. The company should also meet set standards expected of the product. It should offer the product at fair prices to their consumers. In order to meet consumer social responsibility, a company should have a quality control department that checks on the product and service quality. By having a quality control department, the company addresses and ensures consumer satisfaction by producing quality products and services to its customers. Employees provide the company with the workforce or labor needed for creation of products and services in the business. In pursuit of the business missions and goals, social responsibility incorporates employees. A company should be able to ensure that its employee’s needs become met. Social responsibility in ensures that matters such as employee remuneration, bonuses, hiring and training or education get addressed (Werther and Chandler, 2010). A company should ensure that its employees get paid in time and according to labor laws. The company should be able to meet the required labor laws. The company should also enhance employee training and education. Employees should get to attend workshops and seminars to gain more knowledge in order to produce quality products and services. Hiring of employees should be conducted as par the companies required rules and regulation and only qualified candidates should become hired. To ensure that employee social responsibility is met within the business, the company should set up a human resource department which should look up at all matters concerning employees. Stockholders refer to persons or corporation that own shares or equity within a company. The expectation of a stockholder is that the company makes a return on investment (Werther and Chandler, 2010). Stockholder may also be referred to as shareholders or owners of the company. Social responsibility in business gets concerned with ensuring that the expectations of stockholders are met. To ensure that the stockholder s get return on their investment, the company should seek at ways of maximizing its profits. To ensure maximum profits, the company should employ competent management that runs the company smoothly and ensure that it competes fairly and effectively. The company management becomes encompassed with the responsibility of ensuring that all departments work hand in hand to ensure that the company is successful financially to maximize profits. Profits become

Oral history archive department in modern libraries, Essay Example for Free

Oral history archive department in modern libraries, Essay The main aim of the research is to discuss the uses and the value of an oral history archive department in modern libraries, especially British libraries and American ones which are pioneers in this domain. It has been realized or discovered that the oral history archive can be used to explain the concept or idea of windowing time in physical and digital libraries. Of late digital and oral history archive libraries carry a variety of useful information and documents that include the past and present ones. This paper therefore tries to view the use and the value of this kind of sound archive in which it is divided into main categories and subsections. The first section is the introductory part that tries to give a brief explanation about the oral history archive. There is a problem in question or objective, which touches on what is to be investigated that is the use of this archive. The hypotheses are also constructed to provide answers to the objectives of the research. The other areas include research context/literature review; methodology; work plan; recourses; ethics and conclusion. 1. Working Title The use and the value of an oral history archive department in Modern Libraries and Information Centres. 2. Introduction. Many scholars realized that Oral history archive libraries can be used to obtain information as fast as possible. According to Portelli (1998), the archive holds several hours of gramophone, films, audio, video and Compact Disks. The oral history archive has been of great help in various departments because instant information has been obtained through what is called oral debriefing. This is a situation whereby a comprehensive and condensed verbal testimony is obtained from individuals (Medical Tribune 2007). Other areas where the oral history archive has been applied include areas such as psychology, witness interrogation in criminal investigations, which occur in both oral and visual formats (Sangster 1998).The archive tentatively is currently being used to help in knowledge management especially when it comes to labor market. This is an area in commerce and industry where the changing labor market requires employees to shift from one position to another or from one job to another. As this happens there is loss of organizational memory, the so called the body of data and knowledge which is relevant to an individual organization’s existence (Perk and Thomson 1998 and Gregory 1991). These therefore are of the uses of oral history archive that make it become recognized as a powerful device or tool especially in Britain to capture the already existing institutional knowledge and information. Learning has been enhanced by the use of Oral history archive. The new curriculum requires students to have sound and good communication skills that prepare them to answers questions either during interviews as they look for jobs after schooling or in classroom (Rolph 1998). Criminal investigation department also employ the use of an Oral history archive when they are interrogating criminals. 3. Aims and objectives In this section, the paper discusses the problem in question or the objectives of carrying out the research. Oral history archive has been viewed as a unique and powerful tool by people. In order to clear this perception and make things more clear, this research paper tries to answer the question. The problem to be researched on is therefore the value and the use of an oral history archive in modern libraries and information centres and why people view it as a useful tool in modern libraries. The paper shall also look at the role, if any, of the information specialist of the future in an oral history department and the skills the information specialist must have to operate in such a department. Besides, the paper shall also look at and address the question of how interviewing targets are chosen by the oral history department, how expensive it is to put up an oral history department from scratch at the various libraries, why people view it as a useful tool in the modern libraries and the skills that are required by an information specialist in order for him/her to be able to operate in the department. Today, many educationists are being encouraged to introduce oral history as a powerful tool for serious scholarships in all universities in order to encourage learning. However, there are a number of universities that have introduced and developed extensive collections of oral history while others have not realized the usefulness of the library. For example; Harvard, Princeton and University of California have taken the lead in the development and introduction of oral history archive (Gowers 2006). In addition, the university libraries mentioned above in this domain have operated smoothly from 1955, the British Library has been the pioneer for a lot of researchers of this topic. The reason is simple. The Sound Archive Department of British Library at the present time holds over a million discs, One hundred and eighty five thousand audio tapes, and a lot of video and sound records. These collections which include written literature, dramatized stuff, and music recordings plus sounds of wildlife are collected from across the world. They entail cylindrical equipment created in the nineteenth century to the most recent Compact Disks, Digital Versatile Disk and mini discs. 4. Scope and Definition In order to discover the use and the value of an oral history archive department and to create a sensible project in range and time, the scope of the project will focus mainly on the opinions of curators, librarians and others who are working in this sector. It is important though to be aware that this will emphasize basically on personal viewpoints and changing theories and therefore might not provide a perfect answer. It will also be necessary for the research to stay neutral and determined on the questioners and not go beyond the objectives set as those working in these departments will be more involved with the issues concerning their departments than be drawn to the personal issues. 5. Literature Review The literature review section tries to look at what other scholars have discussed about the same topic to be researched on. This is very important since it avoids or solves cases of duplication of other people’s work. Since other people or scholars at one time had realized the importance of Oral history archive in the modern libraries, it is therefore in order to access their ideas on the topic. The oral history archive has been viewed as a useful tool in a number of areas such as psychotherapy, witness interrogation in crime investigation and learning institutions that are provided in both oral and visual formats (Rolph 1998, Gregory 1991, Parker 1999 and Sarah 2004). According to Ritchie (2003), oral history has useful information or materials that are often ignored by librarians. This is also sometimes overlooked in relation to cost value calculation (Swain 2003). The archive has played a key role in helping in audio collection, attitude and value clarification as well as necessitating multi-media approach to learning (Christel 2006 and Thomson 2000). The most obvious area where oral history archive has been used according to Perks and Thompson (1998) is in learning institutions. This is a case where a tape recorder is used in classrooms to help students understand the concept being taught in class room. However, it has been noticed that a tape recorder has not been effectively and efficiently used as compared to the use of movie, slide or overhead projectors (Lukenbill 2002). One advantage when one uses a tape recorder is that it can be stopped anytime especially when the instructor wants to emphasize important points or to allow urgent questions arising (Thompson 2000). Tapes can also be replayed to enhance re-examining or revisiting and requesting of any order that is desirable. Proper understanding of the lesson is therefore emphasized. Since the new curriculum requires students to have skills in understanding comprehension, analysis and evaluation. Oral history has been of great use to check and examine all these concepts. It is also noted that oral communication skills are very important when it comes to times of interviews where questions are phrased (Swain 2003). The interviewer and the interviewee have to be clear and precise especially in calling for specific information. The questions asked especially during the interviews need also to be answered in the most accurate manner as possible (Ritchie 2003). According to Perks and Thompson (1998), accuracy actually plays a major role in interviews and this can be facilitated by the use of oral history archive. The concept is also emphasized by Seale (2004). As a way of facilitating learning, oral history archive through the use of tapes has facilitated immediate feedback during teaching in schools. This has been done in connection with other secondary sources like books more so in secondary schools. Students’ needs are addressed as soon as possible. This rules out cases of negative delays which is commonly experienced in the traditional scholarly research where numerous collections are required (Thompson 2000). A good teacher who knows how to use tapes only needs to locate appropriate tapes in which he is expected to prepare summarized notes according to students’ understanding. This should be done after testing the entry behavior of the students before opting to use the tapes for instruction (Parker 1999). Oral history archive has also been helpful in clinical libraries (Schacher 2001). Clinicians when giving psychotherapy to patients who are mentally disturbed have used the information contained in the Oral history archive. Such patients may be suffering from Schizophrenia or psychosis (Schacher 2001 and Thompson 2000). As patients talk about their illness the clinician is able to come up with appropriate measures to provide assistance.

History of Germany Essay -- essays research papers

Germany, a country rich in culture and heritage, yet plagued by the fallout of World War I and World War II, has progressed to become the centerpiece of the European Union and the world’s third richest economy. The first German Empire dates back to the Roman Empire starting in the 8th century AD. During the Middle Ages the German Empire fended off many attacks against their soil from the Hungarians and the Slavs. Fighting and power struggles continued until the 1400’s, when the modern world gradually came into existence with intellectual, economic and political changes. During the late 1490’s and early 1500’s, Maximilian I put into motion his plan to reform the German Empire by creating an Imperial Supreme Court (Reichskammergericht), levying imperial taxes and increasing the power of the Imperial Diet (Reichstag) (Wikipedia). By the mid 1500’s Germany was a reforming nation with revolts, uprisings and a general division of the empire based on religious beliefs and resentment. This division of factions led to the Thirty Year’s War which ravaged Germany from 1618 to 1648. The war ended in 1648 with the signing of the Peace of Westphalia. As a result, German territory was lost to France and Sweden. In the following years, imperial power declined as states gained more power. This power struggle led to a century of resistance against German rule and persecution. The French revolution sparked a new war between France and its Eastern neighbors. In 1803 Napoleon relaunched the war against the Roman Empire and abolished almost all the smaller secular states and most of the imperial free cities (Wikipedia). The Roman Empire was formally dissolved on August 6, 1806 when the last Holy Roman Emperor Francis II resigned. At that time the Confederation of the Rhine was established under Napoleon’s protection and in 1815 Napoleon was defeated at Waterloo. Following Napoleon’s defeat, the territory of the former Roman Empire was broken into a loose union of 39 states with 35 ruling princes and 4 free cities. Germany began to be industrialized in the 19th century. In 1825 the first steamship sailed on the Rhine and in 1833 the first telegraph was constructed. Railway lines were built in 1835 and in 1866 Siemens constructed the first dynamo (Wikipedia). The industrialization led to modern warfare techniques and ultimately the nationalism, imperialist competition and mi... ...est Berlin (Wikipedia). West German Chancellor Helmut Kohl outlined a plan for the unification of the two nations on November 28, 1989. A formal union of the two political systems occurred in October, 1990. In essence, East Germany was annexed by West Germany and took on its monetary and legal systems. Currently, Germany is quite possibly the centerpiece of the European Union and a strong support of the enlargement of NATO and the EU. Germany’s military participate in multinational relief efforts and has backed the United States in Afghanistan after the terrorist attacks on 9/11. With annual exports of $900 billion, Germany is the World’s leading exporter, well ahead of the United States. Even with its tarnished past and history of war and struggles, Germany is a country that has come a long way in the last two decades with respect to its government, industry and education. Detwiler, Donald S. Germany: a Short History. Carbondale: Southern Illinois University Press, 1999. â€Å"History of Germany.† Wickipedia. 4 Aug. 2005. 28 Jul 2005 â€Å"History of Germany since 1945.† Wickipedia. 24 Jul 2005. 28 Jul 2005 K., Joseph & G., Owen. A History O’Germany. 7 Feb 2001. 28 Jul 2005

Effects of Social Networking

Social networking has become a well-known to all and all in recent years. People are able to meet and make friends, even if they do not know each other. Moreover, they are able to get updates from their loved ones by using social networking sites. Some people are also discussing different important topics on them. They exchange their ideas as well. However, there are several negative effects of social networking sites on our day to day life. People are becoming socially backward by using social networking sites. They do not like to go out to interact with other people.Logging into a community, they intermingled with other people online. They have also replaced the actual procedure of social interaction. Before the invention of these sites, people were keen to go out and meet with other people, but now they sit in front of computers to relate with others. Furthermore, people who are shy in public take advantage of using communal sites. Thinking beyond the social network should be hard for them. These sites are not helping them to overcome their shyness. If they tried to interact with people, they might make it.But because of these sites, they do not even try to do it. Thus social networking sites make people unsocial and socially reluctant. Another effect of community networking is online predators. If someone uploads photos and videos for showing their friends and families, they could be caught by online hunters. Raiders collect photos and videos that are not theirs and misuse them; for example, by creating rumors. These can be displayed for even teenagers and children to see, causing harm. Children and teenagers are becoming addicted watching adult photos and videos.Those predators also follow what you are doing online. Another problem is social networking sites use internet slang. Thus social networking sites are making people morally unhealthy. Finally, the most important effect is people are losing their valuable time, specially the students and officials. Instead of studying, they are playing on different shared interacting sites. A statistic shows that about 68. 4% undergraduate students spend 11 hours per day by chatting and playing games in social networking sites.Furthermore, officials use these sites instead of doing their job. Social networking sites are being banned because of the inefficiency of the workers. Another statistic shows that about 43% American service holders are addicted to facebook. Social networking leads numerous adverse impacts on our life. People are becoming hesitant as they do not go outside to meet people. They are losing their precious time by surfing social networking sites and chatting with others. People are becoming morally corrupted as a result of using jargons and watching uncensored photos and videos.

‘Blackout’ by Roger Mais Essay

‘Blackout’ is a short story by Roger Mais. It is set in Jamaica and is about racism and the contrast of two different races, sexes and cultures! The story starts off explaining the blackout in the city and the general atmosphere of uncomfortable and tense over the city. At this point the story builds an expectation of some sort of conflict. An American women was waiting at a bus stop. Suprisingly she was not bothered by the darkness, and she was not nervous. A black man slowly approaches her and asks for a light for his cigarette. As she does not have matches she offers her cigarette and as he thanks her she flicks the cigarette away. She does that because she is disgusted that a Black man touched her cigarette and therefore she doesn’t want to smoke it anymore. After the flicking, there’s a moment of discomfort and she asks him why he was still there. He replies with an apology as a comment on her action. He stays and keeps talking about her apparent wealt h and as he talks she becomes more uncomfortable. The conversation between the two then focuses on gender and race. At that moment the reader can sense that actually the woman is interested in the current situation and she might actually be looking for an adventure, but he tells her that she is not his type of women which undermines her. During the conversation the reader can also see that the woman has some very racist thoughts. After a while he sees the bus coming and points at it. She gets on the bus and as it starts moving, she urges herself to look back at him and challenge her prejudices, but thinking of the society and worrying about how unacceptable it would seem she can’t succeed and doesn’t look back while the man picks up the cigarette from the gutter. During this short story there is always this feeling of menace and some kind of threat which is created by the blackout and the odd conversation between the two. This feeling is created especially at the beggining, introduction of the story when the blackout and the loneliness were being described by Mais. He used words and phrases such as; wave of panic, bands of hooligans roaming the streets after dark and assaulting unprotected women, slinking black shadow, to reinforce his point. Telephone Conversation ‘Telephone Conversation’ by Wole Soyinka, the poet talks about two people on the phone and the story goes on to narrate how the African man is looking  for a house and the land lady has proposed a considerable price for the same. The poem strikes a positive note as the man gets to know that his privacy won’t be hampered as the landlady doesn’t stay on the premises. The African man is happy to know that and just before he makes up his mind to consider the offer, he drops in to mention that he is black. On the other end of the line, there was nothing but silence which the African man takes it to be an impolite gesture of refusal. However, the silence is soon broken as the landlady starts to speak again asking him to explain exactly how dark he is. First, the man think that he might have misheard the question but when the landlady repeats, he understands that this is something very important for her to know before she allows him to rent her house. This is so mething that came out entirely devastating for the African man and for a moment he felt disgusted with the question and fancies himself to be a machine, like the phone and that he has been reduced to being a button on the phone. He could also smell the foul from her words and he sees â€Å"red’ everywhere all around. The idea of Telephone conversation is to depict how brutal it can be for a man who is subjected to racial discrimination. The Afro-American man is reduced to shame by the sudden silence from the other side and he gets into a state of make belief where he sarcastically thinks that the lady broke her silence and gave him option to choose and define ‘how dark† he is. â€Å"Like chocolate, or dark or light?† Then, he goes on to answer that he is defined as â€Å"West African sepia† in his passport. The lady not knowing how dark it could be didn’t want to embarrass the man further by resorting to silence. So, she asks him to define what he means. The man replies, that it is almost similar to being a brunette but a dark brunette. All this while, the man has been holding on to codes of formality which breaks loose at the landlady’s insensitiveness. The African man now shouts out loud saying that he is black but he is not that black for anyone to be put to shame. He also says that the soles of his feet and the palms of his hand are all white but he is a fool that he sits on his rear which has turned black due to friction. He knows that the landlady will never be convinced with his black complexion and he senses that she might slam down the receiver on him. At such a crucial juncture, he makes a desperate and silly attempt to plead her to come and take a good look at him but couldn’t help the situation from getting worse. Finally, the landlady slams down the  receiver on his face. Harlem ‘Harlem’ by Langston Hughes reflects the post World War II mood of many African Americans. The Great Depression was over, the war was over, but for African Americans the dream, whatever particular form it took, was still being deferred. Whether one’s dream is as mundane as hitting the numbers or as noble as hoping to see one’s children reared properly, Langston Hughes takes them all seriously; he takes the deferral of each dream to heart. Harlem simply asks, and provides a series of disturbing answer to the questions, â€Å"what happens to a dream deferred?† A closer reading reveals the essential disunity of the poem. It is a ground of unresolved conflict. Five of the six answers to the opening questions are interrogative rather than declarative sentences. The ‘dream deferred’ is the long- postponed and frustrated dream of African Americans; a dream of freedom, equality, dignity, opportunity and success. This poem concentrates, on possib le reaction to the deferral of a dream. The whole poem (Harlem) is built in the structure of rhetoric. The speaker of the poem is black poet. Black people were given the dreams of equity and equality. But these dreams never came true. Despite legal, political and social consensus to abolish the apartheid, black people could never experience the indiscriminate society. In other worlds, their dream never came true. Blacks are promised dreams of equality, justice, freedom, indiscrimination, but not fulfilled. They are delayed, deferred and postponed. Only promissory note has been given but has never been brought into reality.The speaker rhetorically suggests that the dreams will explode and destroy all the limitations imposed upon them. After that the society of their dream will be born. When the dream is postponed or deferred or delayed, it brings frustration, it dries up like a raisin in the sun but there is wet inside, likewise it stinks like rotten meat, it becomes fester like a sore and one day it will explode and cause larger social damages. The poem is in the form of a series of questions, a certain inhabitant of Harlem asks. The first image in the poem is â€Å"dream dries up like a raisin†. The simile likens the original dream to a grape, which is sound, juicy, green and fresh since the dream has been neglected for too long, it has probably dried up. The next image in the poem â€Å"fester like† a sore and then run† conveys a sense of infection and pain. Comparing the  dream to a sore of a body, the poet suggests that unfulfilled dreams become part of us, like a longstanding injury that has gathered pus. The word â€Å"fester† connotes something decay and â€Å"run† literally refers to pus. From this viewpoint of the speaker, this denotes to the pain that one has when one’s dreams always defers. A postponed dream is like a painful injury that begins to be infected. The next image â€Å"Does it stink like rotten meat† intensified the sense of disgust.

The pharmaceutical company Mylan is facing more po Essays - Biology

The pharmaceutical company Mylan is facing more po Essays - Biology The pharmaceutical company Mylan is facing more political pressure to confront the price hike of EpiPen after 20 US senators, including Elizabeth Warren and Bernie Sanders, sent an open letter on Tuesday to the company criticising its "exorbitantly expensive" price hikes. Reports emerged last week that the company had implemented a series of gradual price increases inflating the price of the drug from $56.64 to $317.82, a 461% increase in cost since Mylan acquired the rights to EpiPen in 2007. During that same time, Heather Bresch, chief executive officer of Mylan, saw her pay rise $2,453,456 to $18,931,068 , a 671% increase . Last week, she sold 100,200 of her shares in the company for more than $5m. EpiPen CEO hiked prices on two dozen products and got a 671% pay raise "The EpiPen auto-injector delivers a life-saving dose of epinephrine to patients suffering from anaphylaxis. Anaphylactic shock can lead to serious injury or death if untreated; thus, making sure the EpiPen is readily available for use is a critical part of life for millions of Americans living with severe allergies," the Democratic senators wrote in the eight-page letter addressed to Bresch. "The EpiPen, however, has become so exorbitantly expensive that access to this life-saving combination product is in jeopardy for many Americans. "Mylan's near monopoly on the epinephrine auto-injector market has allowed you to increase prices well beyond those that are justified by any increase in the costs of manufacturing the EpiPen," the senators wrote. The senators gave Bresch a deadline of 12 September to address seven multi-part questions about the company's "Savings Card" for customers with insurance, patient assistance program, school programs and plans to release a cheaper generic version of the EpiPen in several weeks. They were sceptical of the company's attempts to defuse the row, noting that the price of the planned generic "is still three times higher than the cost of the branded EpiPen in 2007". Democratic senators Tammy Baldwin, Richard Blumenthal, Barbara Boxer, Sherrod Brown, Joe Donnelly, Richard J Durbin, Al Franken, Heidi Heitkamp, Mazie K Hirono, Amy Klobuchar, Patrick Leahy, Edward Markey, Chris Murphy, Jack Reed, Brian Schatz, Jon Tester, Tom Udall and Sheldon Whitehouse also signed the letter. Last week, Hillary Clinton commented on the controversy, announcing a plan to reduce drug price increases . In it, the Democratic presidential nominee wrote that manufacturers "should be required to explain significant price increases, and prove that any additional costs are linked to additional patient benefits and better value". She called on Mylan to reduce the price "immediately". Other lawmakers have already written open letters to both Bresch, who is the the daughter of West Virginia senator Joe Manchin, and the Food and Drug Administration. Senators Susan Collins and Claire McCaskill sent a request for information to Bresch from the Committee on Aging. Iowa senator Chuck Grassley and others asked the FDA for suggestions on " alternatives to the EpiPen ". Connecticut senator Richard Blumenthal sent his own letter in addition to the letter sent on Tuesday. "My office has been contacted by dozens of concerned Connecticut residents, families, and first responders who urgently require your life-saving product but fear that its skyrocketing price has put it out of reach," Blumenthal wrote last week. The Guardian has reached out to Mylan for comment. Genetically Modified Babies BERKELEY, Calif. AN advisory committee of the Food and Drug Administration is set to begin two days of meetings tomorrow toconsider radical biological proceduresthat, if successful, would produce genetically modified human beings. This is a dangerous step. These techniques would change every cell in the bodies of children born as a result of their use, and these alterations would be passed down to future generations. The F.D.A. calls them mitochondrial manipulation technologies. The procedures involve removing the nuclear material either from the egg or embryo of a woman with inheritable mitochondrial disease and inserting it into a healthy egg or embryo of a donor whose own nuclear material has been discarded. Any offspring would carry genetic material from three people the nuclear DNA of the mother and father, and the mitochondrial DNA of the donor. Roughly 1,000 to 4,000 children born in the United States each year will develop

Mollusks essays

Mollusks essays Everything You Ever Wanted to Know about Mollusks Mollusks: soft, squishy, slimy, and in some cases tasty animals. Mollusks have a soft body that may be protected by a hard shell. Some mollusks have hard outer shells, such as snails, while others have a light internal shell (squids) or no shell at all (octopuses). There are three characteristics that all mollusks have in common. First of all, mollusks have a mantle. This is their soft outer tissue layer. The mantle, in some species is what produces the hard outer shell. Secondly, mollusks have a muscular foot, which they use to move or hang on. The muscular foot appears in different ways on different mollusks. Third, between the mantle and the foot, there is the visceral mass. Most of the internal organs are located here. There is one more characteristic that most, but not all mollusks share. This is an open circulatory system in which blood flows freely over body tissues and organs. Although not all mollusks have separate sexes, they all reproduce sexually. Reproduction can involve internal or external fertilization. Internal fertilization occurs when a male mollusk transfers sperm into the female mollusk. After the eggs are fertilized, the female deposits them in a safe place for them to grow. External fertilization occurs when two mollusks release sperm and eggs into the water at the same time. The four most well known classes of mollusks are Cephalopoda, Gastropoda, Polyplacophora, and Bivalvia. Class cephalopod includes nautilus, squids, cuttlefish, and octopuses. Modern cephalopods are very much the same as their ancestors. They have very developed nervous systems and a closed circulatory system. Gastropods include mollusks such as snails, slugs, and conches. To feed, gastropods scrape algae, shred leaves, or capture prey with their radula. Some gastropods have destroyed millions of dollars in crops. Polyplacophoras most closely resemble ancient mollusks. Their shell i...

Teaching Strategies to Promote Student Equity

Teaching Strategies to Promote Student Equity Designing a classroom learning environment where all students are being attended to (even the ones who may not seem to be engaged) may seem like an impossible task when you are in a classroom of twenty elementary students. Luckily, there are a host of teaching strategies that foster this type of learning environment. Sometimes these strategies are referred to as equitable teaching strategies or teaching so that all students are given an equal opportunity to learn and thrive. This is where teachers teach to all students, not just the ones that seem to be engaged in the lesson.​ Often times, teachers think they have designed this wonderful lesson where all students will be willfully engaged and motivated to participate, however, in actuality, there may only be a few students who are engaged in the lesson. When this happens, teachers must strive to structure their students learning environment by providing a place that maximizes fairness and allows all students to equally participate and feel welcomed in their classroom community. Here are a few specific teaching strategies that elementary teachers can use to promote student engagement and foster classroom equity. The Whip Around Strategy The Whip Around strategy is simple, the teacher poses a question to his/her students and gives every student the opportunity to have a voice and answer the question. The whip technique serves as an important part of the learning process because it shows all students that their opinion is valued and should be heard. The mechanics of the whip are simple, each student gets about 30 seconds to respond to the question and there is no right or wrong answer. The teacher whips around the classroom and gives each student the chance to voice their thoughts on the given topic. During the whip, students are encouraged to use their own words to describe their opinion on the set topic. Often times students may share the same opinion as their classmates but when put into their own words, may find out their ideas are actually a little different than they first thought.   Whips are a useful classroom tool because all students have an equal opportunity to share their thoughts while actively being engaged in the lesson. Small Group Work Many teachers have found integrating small group work to be an effective way for students to equally share their thoughts while staying engaged in the lesson. When educators structure opportunities that require students to work together with their peers, they are giving their students the best possible chance for an equal learning environment. When students are placed in a small group of 5 or fewer individuals, they have the potential to bring their expertise and thoughts to the table in a low-key atmosphere. Many educators have found the Jigsaw technique to be an effective teaching strategy when working in small groups. This strategy allows students to support one another in order to complete their task. This small group interaction allows all students to collaborate and feel included. Varied Approaches As we all know now after must research, all children do not learn the same or in the same way. This means that in order to reach all children, teachers must use a variety of approaches and techniques. The best way to teach equitably to a large number of students is to use multiple strategies. This means that the old singular teaching approach is out the door and you must use a variety of materials and strategies if you want to meet all learners needs. The easiest way to do this is to differentiate learning. This means taking the information that you know about the way each individual student learns and using that information to provide students with the best possible lesson. Studies have shown that using different strategies and techniques to reach different learners is the best possible way that teachers can cultivate a classroom of equity and engagement. Effective Questioning Questioning has been found to be an effective strategy to promote equity and make sure all students are actively being engaged. Using open-ended questions is an inviting way to reach all learners. While open-ended questions require some time to develop on the teachers part, it is well worth it in the long run when teachers see all students actively and equally being able to participate in classroom discussions. An effective approach when using this strategy is to give students time to think about their answer as well as to sit and listen to them without any interruptions. If you find that students have a weak answer, then pose a follow-up question and continue to question students until you are sure they have understood the concept. Random Calling When a teacher poses a question for his/her students to answer, and the same children constantly raise their hands, how are all students supposed to have an equal chance at learning? If the teacher establishes a classroom environment in a non-threatening way where students can be chosen to answer a question at any time, then the teacher has created a classroom of equality. The key to the success of this strategy is to make sure that students do not feel pressure or threatened to answer in any way, shape or form. One way that effective teachers use this strategy is to use craft sticks to call upon random students. The best way to do this is to write down each students name on a stick and place them all into a clear cup. When you want to ask a question you simply pick out 2-3 names and ask those students to share. The reason you choose more than one student is to minimize the suspicion that the only reason the student is being called upon is that they were misbehaving or not paying attention in class. When you have to call upon more than one student it will ease all students anxiety level. Cooperative Learning Cooperative learning strategies are perhaps one of the simplest ways teachers can effectively keep their students engaged while promoting equity in the classroom. The reason being is it gives students the opportunity to share their thoughts in a small group format in a non-threatening, non-biased way. Strategies like think-pair-share where students each take a specific role in order to complete a task for their group and round robin where students can equally share their opinion and listen to the opinion of others gives students the perfect opportunity to share their thoughts and listen to the opinions of others. By integrating these types of cooperative and collaborative group activities into your daily lessons, you are promoting participation in a collaborative versus a competitive way. Students will take notice which will help turn your classroom into one that cultivates equality. Enforce a Supportive Classroom One way teachers can cultivate a classroom of equality is to establish a few norms. A simple way to do this is to verbally address the students at the beginning of the school year and let them know what you believe in. For example, you can say All students are treated with respect and When sharing ideas in class you will be treated with respect and will not be judged. When you establish these acceptable behaviors students will understand what is acceptable in your classroom and what is not. By enforcing a supportive classroom where all students feel free to speak their mind without feeling or being judged you will create a classroom where students feel welcomed and respected.

Molecular Neuro-degeneration Essay Example | Topics and Well Written Essays - 1500 words

Molecular Neuro-degeneration - Essay Example It is evident that no drug has been discovered that could to completely protect neurons; there are two possible approaches to that may come up with the treatment of AD. One approach involves treatment which prevents the onset of the disease through curbing the primary targets and reducing the subsequent pathologies of the AD. This way, it becomes possible to slow the disease progression and hence prevention to the development of AD (Vradenburg 2013). The second approach involves the symptomatic treatment; an approach whereby the primary and tertiary symptoms of the AD are declined. Through this approach, reflection to the current state of treatment including the usual treatment the cognitive impairment, the decline in global function, deteriorating performance of activities of daily living and behavioral change ( Perry 2013). When searching for the appropriate treatment strategies, scientists concentrate on the severity of the disease and also the specificity of each individual. Currently the available therapeutic agents are the main target to specific symptoms of the AD; the agents such as cholinesterase inhibitors involved in the enhancement of cholinergic neurotransmission and also the inhibiting of acetylcholine degradation within the synapse are the Alzheimer’s disease main treatment (Vradenburg 2013). Going by the report on the role of the involvement of PI3 kinase signaling in AÃŽ ²-induced memory loss in Drosophila, the PI3K-Akt-mTOR pathway regulation in AÃŽ ² oligomer neuronal cell cycle process and Amyloid-b Interruption of the PI3K-Akt-mTOR signaling pathway involvement in brain-derived neurotrophic factor-induced arc expression in rat scientists can improve theirs unleash a further superior drug to completely heal the Alzheimer’s disease (Perry 2013). One of the hindrances to the progress of other researchers on AD have been due to the fact that most drugs developed show general success in animal specimens but when testing them to human beings, they become less responsive, Therefore, it is safe to say that this project is still in its infancy and further studies have to be carried out but in the long run a solution will be arrived upon.

Genetic reprogramming holds great promise for the discipline of Research Paper

Genetic reprogramming holds great promise for the discipline of regenerative medicine, because of the ability to obtain patient- - Research Paper Example These cells are similar to embryonic cells in that they can differentiate into the various body cells under favorable conditions. The advantage of iPSCs cells over the embryonic cells is that they do not need embryos during production. This makes the technology ethically acceptable. IPSCs cells production involves inserting of stem cell associated genes into specialized somatic cells using viral vectors (Xiong et al 1). Genetic engineers hopefully believe iPSCs cells will initiate the production of cells or tissue from a patient that will repair the damaged tissues. The cells regenerated through induced pluripotent stem cell method are most suitable in restoring damaged cells and tissues. This is because the patient’s immune system will readily tolerate them. This will eliminate the problem of graft rejection exhibited in xenografts or isografts. Elimination of immune suppressive drugs on patients usually does happen. Despite all these seemingly possible setbacks, induced plur ipotent stem cells have proofed to be the future of the regenerative medicine. Patients suffering from cardiovascular disease and other disorders will find cure after the establishment of this technology. Application of iPSCs in treatment of cardiovascular disease Cardiovascular disease affects the cardiovascular system (the heart and the blood vessels). These diseases include coronary heart disease, stroke, congestive heart failure, pulmonary embolism among others. The iPSCs technology promises a positive break through to the medicine world (Amit &Joseph112). The ability to induce adult specific cells into stem cell without use of embryo enables clinicians to change other cells from a patient. The body recognizes the cells as self and therefore no rejection can occur. Researchers are successfully modeling many cardiovascular diseases by this technology. The improved understanding of the cardiovascular diseases provides a better treatment in the medicine world. Formation of cardiomy ocytes Reprogramming of somatic cells into iPSCs is by integrating or non-integrating method. The integrating method uses viral vector while the non-integrating method uses a plasmid to deliver the genes. For cardiovascular disease treatment, the most suitable is the non-integrating method. Reprogramming of the fibroblasts of the dermis of the patient produces the iPSCs for use. A method called embroyoid body differentiation turns the iPSCs to revolve into cardiomyocytes (Nelson et al 2). The cardiac cells produced this way show the same characteristics of human cardiac cells although they may differ in morphology. To determine functionality of the iPSCs, cardiomyocytes researchers use molecular techniques such as immunocytochemistry and polymerase chain reaction techniques (Amit & Joseph117). The cardiomyocytes formed from patient cells posses the mutations that cause disease in the patient. These cells serve as the model for the disease and therefore further investigations are pos sible. Modeling of inherited cardiovascular diseases is very important in determining its cure. The iPSCs cardiomyocytes (heart muscle cells) provide the best model for these diseases. The iPSCs provide insights to concerns of heart repair. This technology is a potential source of cells for repairing the heart and blood vessels. Studies done by Li and co-researchers gave the evidence that induced pluripotent stem cells would provide unlimited resources for transplantation. This form of tissue

Chapter review Essay Example | Topics and Well Written Essays - 1000 words

Chapter review - Essay Example While the peacebuilding operations have gained pace, the studies with respect to the challenges and operations of peace building have also come into focus. They try to bring about therefore, a globalization of not merely the goods and services but the way a state should function. They promote democracy and free market mechanism. These standards are thus getting transmitted from the western regions (serving as the core) to the periphery (the nations undergoing reconstruction). This is the main point of argument in this research paper. The idea is not to proclaim that such globalization is not appropriate, but to focus on the perspectives of peacebuilding operations leading to promotion of a single kind of framework in terms of economy and administration. In this context, the activities of the peacebuilders might be related to the idea behind ‘mission civilisatrice’, which is the policy of civilizing the dependent countries followed by Europe during the colonial period. Th e analysis followed for the paper is qualitative in nature and secondary research method is followed to empirically show how the peace promoting organizations have promoted the liberal market democracies in the war-shattered nations. In the first section the author presents this empirical analysis and explains the mechanisms through which this process is put into action by the peacebuilders. In the second section the author first connects the idea of peacebuilding and that of ‘military civilisatrice’. Next, he associates peacebuilding operations with globalisation. The paper describes in the empirical analysis, how organizations like US Agency for International Development (USAID), The Organisation of American States (OAS) etc have promoted the western ideologies of liberal market democracy in peripheral countries like Namibia, Angola, Rwanda, Nicaragua, Guatemala, Cambodia,

Gospel Music Features in the Christian Liturgy of the Pentecostal Essay

Gospel Music Features in the Christian Liturgy of the Pentecostal Church - Essay Example James (2001, p. 56) claims that the style of gospel music that exists in any given Pentecostal church depends partly on the age of the singers. Thus, the senior adult tend to prefer the transitional and traditional gospel music while, the young adults prefer modern gospel music that may predominate the main worship service. Whatever the period and age, the singing is given in the form of gospel songs in the Pentecostal churches. Development of gospel music People who were enslaved and worked in the field during the civil war told their stories in the spiritual way explaining their current life and teaching their children on the hardship they endured. When the enslaved adopted the Christian religion, their faith naturally became the focus of their songs. However, their songs were no longer limited to the spiritual because they started using the hymns written by white composers like Isaac Watts and Wesleys. The enslaved accepted the gospel music because it was attributed to their style and culture, especially the meter hymn that is still used in the most Pentecostal churches. As the civil war came to an end and enslaved were finally emancipated, it marked the beginning of what is referred to the historical gospel hymns. According to James (2001, p. 55), these songs flourished during the social and economic deprivation of depression and started to make their way out of the Pentecostal churches. According to Anderson and James (2007, p. 285) indicate that the civil rights movement brought rebirth of older songs, especially spiritual songs that became the freedom songs. These spiritual songs were the bridge between what is known as historical gospel and modern gospel songs. The publicizing of the Pentecostal churches and protest brought a wider exposure to the gospel music and its popularity spread. Currently, the modern gospel is one of the most popular sounds in the music industry. In the twentieth century, the most widespread and celebrated of the people traditio n was the growth and development of gospel music, especially in the Pentecostal churches. These churches were visited by the traveling musicians and evangelists from the Chicago gospel scene like Sallie Martin and Thomas Dorsey. Despite this contact with new gospel sounds, arranged spirituals, and hymns were still popular styles of church music. Pastors and Pentecostal churches supported the expansion of gospel music by starting gospel choirs and hosting outstanding concerts in various Pentecostal churches like opportunity Baptist. Various composers started transcribing and publishing their gospel music and gospel performers protected themselves from exploitation by forming gospel unions. Anderson and James (2007, p. 289) indicate that the gospel growth extended beyond churches in the black community and moved to worldly white venues around the city. Most of the gospel composers and performers received attention via television programs, firms and release of albums by choirs. Thus, t he developed technology has assisted a lot in developing the gospel music worldwide. Many gospel singers have developed new styles of singing and different, exciting music ministries. From neo spiritual to the modern gospel and praise and worship music, these song writers reflect the range of gospel music in Pentecostal churches today. The gospel music composers Wallace (2005, P. 104) indicates that the gospel music was brought substantial change in the Pentecosta

Physics of racing Research Paper Example | Topics and Well Written Essays - 750 words

Physics of racing - Research Paper Example 2). Several formulas will determine the calculations made in the research paper to determine several aspects of the mechanics involved in motorcar racing. Introduction The wide-ranging goal of the research paper is to put forward a new prospect on racing physics, comprehensible to the practically motivated non-specialist (Beckman, 2002, p. 6). There are a number of problems that are frequently regarded from a range of sources, capable of preventing quality results from determining the physics behind racing. Regularly, these issues are inclined by automated simulation, and they regularly come up from contest experiences. This research paper will also get extremely technical, attempting to balance a conceptual discourse that every individual ought to get to comprehend. Through an arithmetic analysis, the following paper may be of great interest just to physicists, and with numerical outcomes that might once more be available to all analysts worldwide. Weight transfer Balancing a vehicl e is directing weight transfer through the use of acceleration, brakes and piloting. Applying the brakes adjusts weight to the anterior part of the vehicle, enabling the driver to prompt over-steering of the vehicle. In a similar fashion, throttling adjusts the weight of the vehicle to the back of the vehicle, prompting under-steering and cornering adjusts weight to the reverse side, unpacking the interior part of the wheels (Beckman, 2002, p. 12). The reason behind this adjustment in the weight of the vehicle is that inertia acts via the center of gravity (CG) of the vehicle, which is on top of the ground level. At the same time, adhesive forces act at ground levels via the touching base spots of the wheels. The impacts of weight transfer are relative to the elevation of the CG from the ground. If a much flatter vehicle with a decreased CG was involved in racing, its handling would be much improved and velocity would be higher because weight transfer is not very extreme in comparis on with a much less flat vehicle. The measurement of inert friction is not precisely a constant. Under driving circumstances, numerous effects are used to decrease inert friction of an excellent autocross wheel to an estimated 1.10G (Beckman, 2002, p. 21). These outcomes are a refraction of the wheel, suspension motion, heat and the inflation compression. Nevertheless, the relativity law still stands sensibly true under these circumstances. When the vehicle is accosting, braking, or throttling at the edge, this implies that the adhesive parameters of the wheels can cause them to unload by the weight transfer’s shifting from sticking to sliding off the ground. Each time a vehicle tilts a little or gets a little sideways, the vehicle will most likely lose the race if the mistake is not made up through recovery of laps. Certain calculations formulas and elements can be used to determine the forces and inclinations vehicles and their drivers can make to succeed in a race. For ins tance, in order to make a right turn, a force indicating the right is obliged to act on the vehicle that steer it away from the unbent line it unsurprisingly attempts to pursue. Should the force remain steady, the vehicle will be inclined to move in a circular manner (Beckman, 2002, p. 29). Basic laws Basically, there are three laws that have to be agreed so as

Gospel Music Features in the Christian Liturgy of the Pentecostal Essay

Gospel Music Features in the Christian Liturgy of the Pentecostal Church - Essay Example James (2001, p. 56) claims that the style of gospel music that exists in any given Pentecostal church depends partly on the age of the singers. Thus, the senior adult tend to prefer the transitional and traditional gospel music while, the young adults prefer modern gospel music that may predominate the main worship service. Whatever the period and age, the singing is given in the form of gospel songs in the Pentecostal churches. Development of gospel music People who were enslaved and worked in the field during the civil war told their stories in the spiritual way explaining their current life and teaching their children on the hardship they endured. When the enslaved adopted the Christian religion, their faith naturally became the focus of their songs. However, their songs were no longer limited to the spiritual because they started using the hymns written by white composers like Isaac Watts and Wesleys. The enslaved accepted the gospel music because it was attributed to their style and culture, especially the meter hymn that is still used in the most Pentecostal churches. As the civil war came to an end and enslaved were finally emancipated, it marked the beginning of what is referred to the historical gospel hymns. According to James (2001, p. 55), these songs flourished during the social and economic deprivation of depression and started to make their way out of the Pentecostal churches. According to Anderson and James (2007, p. 285) indicate that the civil rights movement brought rebirth of older songs, especially spiritual songs that became the freedom songs. These spiritual songs were the bridge between what is known as historical gospel and modern gospel songs. The publicizing of the Pentecostal churches and protest brought a wider exposure to the gospel music and its popularity spread. Currently, the modern gospel is one of the most popular sounds in the music industry. In the twentieth century, the most widespread and celebrated of the people traditio n was the growth and development of gospel music, especially in the Pentecostal churches. These churches were visited by the traveling musicians and evangelists from the Chicago gospel scene like Sallie Martin and Thomas Dorsey. Despite this contact with new gospel sounds, arranged spirituals, and hymns were still popular styles of church music. Pastors and Pentecostal churches supported the expansion of gospel music by starting gospel choirs and hosting outstanding concerts in various Pentecostal churches like opportunity Baptist. Various composers started transcribing and publishing their gospel music and gospel performers protected themselves from exploitation by forming gospel unions. Anderson and James (2007, p. 289) indicate that the gospel growth extended beyond churches in the black community and moved to worldly white venues around the city. Most of the gospel composers and performers received attention via television programs, firms and release of albums by choirs. Thus, t he developed technology has assisted a lot in developing the gospel music worldwide. Many gospel singers have developed new styles of singing and different, exciting music ministries. From neo spiritual to the modern gospel and praise and worship music, these song writers reflect the range of gospel music in Pentecostal churches today. The gospel music composers Wallace (2005, P. 104) indicates that the gospel music was brought substantial change in the Pentecosta

Travelling Essay Example | Topics and Well Written Essays - 750 words - 2

Travelling - Essay Example Moscow and London both are quite famous for travelling. They provide various entertainment packages such as restaurants, galleries, museums, parks, clubs etc. before traveling it is essential to plan everything such as place to visit, expenditure, budget etc. selection of place is based on all these major factors. There are more restaurants in London as compared to Moscow. But these restaurants are very expensive, while the cost of living in Moscow is 39% cheaper as compared to London. This means that a standard of living in London will require $3000. But if a person travels to Moscow one will require just $1830. While the transport network of Moscow is far better than London. In London cinema and other entertainment center are 5% higher than that of Moscow. This shows that traveling to Moscow will be cheaper and beneficial for visitors as compared to London. Travelling provides many benefits to people. It helps to gain experiences and knowledge of various countries because while vis iting to the ancient and architectural places of a country such as Moscow, one can get more knowledge in relation to various systems and culture of a country. It is beneficial for both students as well as parents. They can not only be familiar of one’s culture but will also have a chance to learn new languages. People have many motives to travel such as to discover something new, for pleasure, to explore, for relaxation and to learn other cultures. Therefore, I think one should keep on travelling throughout his or life.

First People of Canada Essay Example for Free

First People of Canada Essay The purpose of the writer is to present his analysis of the present condition of the educational system of Canada which he regards as colonial education for the aborigines of Canada. He examined the manner in which the Indigenous education and epistemologies have been ignored and undermined and made recommendations on the revitalization of an education reflective of the needs and sentiments and culture of the Aboriginal Canada. The article is based on facts and not opinion. The information given are well researched and are supported as the writer presented evidences that the present educational system has â€Å"physically, spiritually and mentally destructive and disruptive components of colonial education (p. 3)† The objective of the author is well achieved as his arguments are logical. His choice of language is effective for his intended audience is the general public especially the education sector who needs to do something about the colonial education. The author discussed that the residential schooling and the Eurocentric schooling and the curriculum are not reflective of the culture of the Aborigines and were far different from the traditional education. The education then is colonial and beyond the experiences and the daily life of the Aborigines. It needs to be reformed. The author is successful in letting his audience understand his point because his illustrations why he takes the education of Canada as colonial are very effective.

1,2,4-Oxadiazole Moiety Molecules Synthesis for Cancer

1,2,4-Oxadiazole Moiety Molecules Synthesis for Cancer 2.4. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole derivatives for their MTT assay using MCF-7 breast cancer cell line and degradation of DNA in EAT cells 2.4.1. INTRODUCTION In the biological and pharmacological importance, heterocycles plays a significance role. Oxadiazole molecules show biologically activity includes angiogenesis inhibitor [246] and also HIV inhibitor [247], tyrosine kinase inhibition [45], histamine H3 antagonism [48], muscarinic agonism [49], potent histamine H2 receptor antagonists [50, 51], muscarinic receptor antagonists [53, 54], interleukin-8 (IL-8) receptor antagonists [65], cytotoxic activities [68], monoamine oxidase inhibition [66], potent therapeutic agents for prostate cancer [72], anticonvulsant activity [67], tumor-selective and apoptosis-inducing agents [70, 71], antitumor [4f] and apoptosis-inducing anticancer agents [73, 74]. Breast cancer is a most terrifying disease in which cells in breast tissue grow and divide without normal control. This type of growth of cells without control forms a lump called tumor. In breast cancer, tumors are called benign or malignant. Malignant tumors will grow by eating food. They get the food by forming new blood vessels in a process called angiogenesis. These blood vessels are the main reason to promote the growth of the tumors. After this tumor growing it will spread to nearby tissue, which is called as invasion. The breakage of main tumor cells will spread into other parts of the body and it will lead to metastatic breast cancer. This happens through blood stream or lymphatic system and this process is called metastasis. The main disadvantage of the malignant breast cancer is dividing and grows out of control which leads to form number of new tumors. If those new tumors are in other parts of the body, then also we call those as breast cancer. Especially in women, breast cancer leading to the cause of cancer related death. In developing and developed countries, breast cancer is the second most common malignancy type diagnosed disease in women. In India breast cancer is the most discussing problem in the current health problem (248). By the survey conceded by the Indian Council of Medical Research (ICMR), the percentage of breast cancer patients has been nearly doubled. In the past few years nearly one lakh new patients are being detected from 1985 to 2001 (249, 250). It has been estimated that the breast cancer in 2004 is nearly 90,273 and they predicted that in 2015 the patient’s number may be nearly 1, 12,680 (251). Due to the damage in DNA, normal cells will become cancer cells. DNA is present in every cell and it directs to all its actions. When DNA gets damaged in normal cells, the cell either repairs the damage or it dies. But in the cancer cells, damaged DNA is not repaired. The damaged cell undergoes splitting. As a result cell goes on making new cells that the body doesn’t need and those cells have same damaged DNA as the first cells does. This conjecture the design and synthesis of new anticancer drugs, and drug combination and treatment modalities is still the need for effective treatment of breast cancer patients [252]. 1,2,4-Oxadiazole moiety molecules show signs of vide variety of biological activities [40, 253-255]. In connection to the above studies, our molecules are subjected to the angiogenesis using MCF-7 breast cancer cell lines and degradation of DNA studies using in EAT cells. 2.4.2. MATERIALS Melting points were recorded (uncorrected) on a Buchi Melting Point B-545 instrument. Infrared (IR) spectra were recorded using a Jasco FTIR-4100 series. All reagents and solvents used were commercially procured and used as received. 1H-NMR spectra’s were recorded on Shimadzu AMX-400-Bruker with 400 MHz with TMS as internal standard. The 13C NMR spectra were examined on a Bruker DPX-400 at 100.6 MHz. The mass spectra were recorded on a JEOL JMS-AX505HA mass spectrometer. 2.4.3. EXPERIMENTAL 2.4.3.1. Chemistry General procedure for synthesis of (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2). A solution of hydroxylamine hydrochloride (1.529 g, 22.004 mmol) (2.5eq) and sodium carbonate (1.492 g, 14.082 mmol) (1.6eq) was taken in a round bottom flask. Stir for 10min to dissolve completely, then to this mixture 4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorobenzonitrile (1) (2.0 g, 8.801 mmol) (1.0 eq) is dissolved with ethanol was added. Then the mixture is heated to 60 0C about 5-6 hr. After that the steps forward of the reaction fusion was examined by the thin layer chromatography (TLC). After reaction completion, the solvent and the product was separated in vacuum pump under reduced pressure. Then the product was poured to water and extracted with ethyl ethanoate. The organic layer was washed 2-3 times with distilled water. The organic layer was washed 2-3 times with distilled water. The extracted ethyl ethanoate layer was dried over sodium sulphate (anhydrous) and the solvent was evaporated to get (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hyd roxybenzimidamide (2). 2.4.3.2. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole 4(a-f) derivatives. (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (1.0 eq) is dissolved in dry dichloromethane and cooled to 0-5 0C in ice bath. Then N,N-diisopropylethylamine (1.1 eq) was added to cold reaction mixture and stirred for 10 minutes, then different aromatic acid chlorides (3a-e) (1 eq) were added. The reaction mixture was allowed to room temperature under stirring for 5-6 hr. After that the steps forward of the reaction fusion was examined by the thin layer chromatography (TLC). After reaction completion, the solvent and the product was separated in vacuum pump under reduced pressure. Then the product was poured to water and extracted with ethyl ethanoate. The organic layer was washed 2-3 times with distilled water. The organic layer was washed 2-3 times with distilled water. The extracted ethyl ethanoate layer was dried over sodium sulphate (anhydrous) and the product was purified with the help of column chromatography over silica gel (60-120 mesh) using hexane and ethyl acetate (1:1). Scheme 1. Reagents and conditions: (i) Sodium carbonate, water, ethanol, 60 0C, 6 h; (ii) dichloromethane, N,N-diisopropylethylamine, 0-5 0C, 6 h; 3(a-e) Where 3a = 4-chloro benzoyl chloride; 3b = 4-Fluoro benzoyl chloride; 3c = 4-(trifluoromethyl)benzoyl chloride; 3d = 4-Fluoro-3-Nitrobenzoyl chloride; 3e = 4-EthylPhenylbenzoyl chloride. 2.4.3.2.1. Synthesis of 5-(4-chlorophenyl)-3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-1,2,4-oxadiazole (4a) Pale yellow color from (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (0.1 g, 0.384 mmol), 4-chlorobenzoylchloride (3a) (0.067 g, 0.384 mmol) and N,N-diisopropylethylamine (0.049 g, 0.461 mmol). 1H NMR (400 MHz, CDCl3): 8.32 (d, 1H, Ar-H), 7.75 (dd, 2H, Ar-H), 7.70, (d, 1H, imid-H), 7.55 (d, 1H, Ar-H), 7.50 (dd, 2H, Ar-H), 7.35 (d, 1H, imid-H), 7.30 (d, 1H, Ar-H), 5.05 (d, 1H, pyrrole-H), 2.56-2.30 (d, 4H, pyrrole-H); MS (ESI) m/z: 381.081 (100.0%), Anal. calcd. for C20H14ClFN4O (in %): C- 63.08, H- 3.71, N- 14.71. 2.4.3.2.2. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (4b) Orange color from (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (0.1 g, 0.384 mmol), 4-Fluoro benzoyl chloride (3b) (0.060 g, 0.384 mmol)and N,N-diisopropylethylamine (0.049 g, 0.461 mmol). 1H NMR (400 MHz, CDCl3): 8.31 (d, 1H, Ar-H), 7.30 (dd, 2H, Ar-H), 7.72, (d, 1H, imid-H), 7.56 (d, 1H, Ar-H), 7.34 (d, 1H, imid-H), 7.31 (d, 1H, Ar-H), 7.29 (dd, 2H, Ar-H), 5.02 (d, 1H, pyrrole-H), 2.58-2.31 (d, 4H, pyrrole-H); MS (ESI) m/z: 365.114 (100.0%), Anal. calcd. for C20H14F2N4O (in %): C- 65.93, H- 3.87, N- 15.38. 2.4.3.2.3. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-(4-(trifluoromethyl)phenyl)-1,2,4-oxadiazole (4c) Dark brown color from (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (0.1 g, 0.384 mmol), 4-(trifluoromethyl)benzoyl chloride (3c) (0.080 g, 0.384 mmol) and N,N-diisopropylethylamine (0.049 g, 0.461 mmol). 1H NMR (400 MHz, CDCl3): 8.33 (d, 1H, Ar-H), 8.10 (dd, 2H, Ar-H), 7.74 (d, 1H, imid-H), 7.70 (dd, 2H, Ar-H), 7.58 (d, 1H, Ar-H), 7.37 (d, 1H, imid-H), 7.33 (d, 1H, Ar-H), 5.06 (d, 1H, pyrrole-H), 2.59-2.29 (d, 4H, pyrrole-H); MS (ESI) m/z: 415.110 (100.0%), Anal. calcd. for C21H14F4N4O (in %): C- 60.87, H- 3.41, N- 13.52. 2.4.3.2.4. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-(4-fluoro-3-nitrophenyl)-1,2,4-oxadiazole (4d) Pale yellow color from (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (0.1 g, 0.384 mmol), 4-Fluoro-3-Nitrobenzoyl chloride (3d) (0.078 g, 0.384 mmol)and N,N-diisopropylethylamine (0.049 g, 0.461 mmol). 1H NMR (400 MHz, CDCl3): 8.71 (d, 1H, Ar-H), 8.65 (d, 1H, Ar-H), 8.34 (d, 1H, Ar-H), 7.74 (d, 1H, imid-H), 7.61 (dd, 1H, Ar-H), 7.58 (d, 1H, Ar-H), 7.37 (d, 1H, imid-H), 7.33 (d, 1H, Ar-H), 5.06 (d, 1H, pyrrole-H), 2.59-2.29 (d, 4H, pyrrole-H); MS (ESI) m/z: 410.099 (100.0%), Anal. calcd. for C20H13F2N5O3 (in %): C- 58.68, H- 3.20, N- 13.52. 2.4.3.2.5. Synthesis of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-(5-ethyl-[1,1-biphenyl]-2-yl)-1,2,4-oxadiazole (4e). White color from (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) (0.1 g, 0.384 mmol), 4-EthylPhenylbenzoyl chloride (3e) (0.094 g, 0.384 mmol) and N,N-diisopropylethylamine (0.049 g, 0.461 mmol). 1H NMR (400 MHz, CDCl3): 8.31 (d, 1H, Ar-H), 7.95 (d, 1H, Ar-H), 7.80 (dd, 2H, Ar-H), 7.75 (d, 1H, Ar-H), 7.72, (d, 1H, imid-H), 7.53 (dd, 2H, Ar-H), 7.56 (d, 1H, Ar-H), 7.45 (d, 1H, Ar-H), 7.34 (d, 1H, imid-H), 7.30 (d, 1H, Ar-H), 7.31 (d, 1H, Ar-H), 5.03 (d, 1H, pyrrole-H), 2.65 (q, 2H, -CH2), 2.58-2.31 (d, 4H, pyrrole-H), 1.27 (t, 3H, -CH3),; MS (ESI) m/z: 451.186 (100.0%), Anal. calcd. for C28H23FN4O (in %): C- 74.65, H- 5.15, N- 12.44. 2.4.4. Biology 2.4.4.1. Culture of MCF-7 cells: MCF-7 cells were cultured with minor modification in Minimal Essential medium (Invitrogen) supplemented with 10% fetal bovine serum, 100units/ml penicillin-G, 100  µg/ml streptomycin and 1% sodium bicarbonate (Invitrogen). MCF-7 cells were obtained from Cell repository unit of National Center for Cell Sciences (NCCS), Pune, India. All cell lines were maintained at 37 °C in a humidified atmosphere with 5% CO2 [256]. 2.4.4.2. Culture of EAT cells: Animals, in vivo tumor generation and imidazole derivatives treatment Six to eight weeks old female mice were acclimated for one week while caged in-group of five. Mice were housed and fed a diet of animal chow and water ad libitum throughout the experiment. All the experiments were approved by the institutional animal care and use committee of the University of Mysore, Mysore, India. Ehrlich Ascites Tumor (EAT) cells (5Ãâ€"106 cells/mouse) were injected intraperitoneally. These cells grow in mouse peritoneum forming an ascites tumor with massive abdominal swelling. The animals showed a dramatic increase in body weight over the growth period and the animals succumbed to the tumor burden 14–16 days after implantation. 2.4.4.2.1. Isolation of EAT cells from mice peritoneal cavity and compound treatment: From the peritoneal cavity of tumor-bearing mice the EAT cells were isolated (control and treated). 2-3 mm of sterile PBS was injected in to the peritoneal cavity of the mice and the peritoneal fluid containing tumor cells withdrawn, collect in sterile petri dishes and incubated at 370C for 2 h. The cells of macrophage linage adhered to the bottom of Petri dishes. The non-adherent population was aspirated out gently and washed repeatedly with PBS. Moreover, viability of these cells was assessed and was found to be >95% by trypan blue dye exclusion. The viable EAT cells were processed for further experiments. The EAT cells (5 x 106) were treated with or without compounds of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole series 4(a-e) and incubated at 370 C for different time interval or for known period of time. After the incubation period the cells w ere used for the further analysis [258]. 2.4.4.2.2. Cell count by Trypan blue dye exclusion assay. EAT cells were treated with different concentrations of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole compounds 4(a-e) at various time periods (0–4 h). Cell viability was assessed by mixing aliquots of cell suspension with 0.4% trypan blue and counted using heamocytometer. Cells that picked up the dye were considered to be dead [259(a)]. 2.4.5. Result and Discussion 2.4.5.1. Chemistry Synthesis of the key intermediate (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2) is outlined in Scheme 1. Briefly, hydroxylamine hydrochloride and sodium carbonate was taken in water and stirred. 4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorobenzonitrile (1) was dissolved in ethanol and added to the reaction mixture. The presence of –NH2 and =N-OH proton peaks NMR spectra indicates the formation of (Z)-4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluoro-N-hydroxybenzimidamide (2). The key intermediate compound (2) was taken in dry dichloromethane and cooled to 0-5 0C, and N,N-diisopropylethylamine was added. Stirred for 10 min, then different aromatic acid chlorides 3(a-e) was added drop by drop. The reaction mixture was allowed to room temperature under stirring for 5-6 h and after that the steps forward of the reaction fusion was examined by the thin layer chromatography (TLC). After reaction completion, the solvent and th e product was separated in vacuum pump under reduced pressure. Then the product was poured to water and extracted with ethyl ethanoate. The organic layer was washed 2-3 times with distilled water to get target 3-(4-(3-(1H-imidazol-5-yl)propyl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole 4(a-e). Upon completion crude products 4(a-e) were obtained with a good yield of 81–93% and which the product was purified with the help of column chromatography over silica gel (60-120 mesh) using hexane and ethyl acetate (1:1). The absence of –CO proton peak in synthesized derivatives in 1H spectra confirmed the identity of the products. The details of chemical structures, physical data and purity of compounds are given in Table 1. Compound R1 Yield MP (oC) Purity 4a 90 277 90 4b 85 100 93 4c 81 110 89 4d 82 142 92 4e 79 95 81 Table 1. Chemical structures, physical data and purity of compounds 4(a–e) 2.4.5.2. Biology 2.4.5.2.1. MTT assay: The MTT assay was performed according to the protocol previously reported [257]. MCF-7 cells were plated at a density of 1 X 105 cells in 96-well plates. (Subsequently, the 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole series 4(a-e) were assayed using concentrations from 0.05 to 0.5 mM). After 24 h of incubation, 10  µL of 5% 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Sigma Aldrich) (0.05 mg/mL) were added to the culture medium. After 4 h at 370C the culture medium was removed and 200  µL of DMSO were added to dissolve the salts of formazan. The absorbance was measured with a 96-wells plate spectrophotometer at 570 nm. The experiments were independently performed three times and each experiment contained triple replicates. Control samples containing a complete culture medium devoid of cells or control cells with 0.1% DMSO were also included in each experiment. Figure 1. The MTT assay of compounds 4(a-e) in MCF-7 breast cancer cell lines. Sl.No. Name of the compound IC50 Value 1 Cisplatin 10ÃŽ ¼g 2 4a 100ug 3 4b 200ug 4 4c 100ug 5 4d 800ug 6 4e 200ug Table 2. Compounds 4(a-e) and their IC50 value ( µg/ml) on MCF-7 breast cancer cell lines. 2.4.5.3. DNA fragmentation assay: EAT cells were collected from mice treated with or without 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazole series 4(a-e). Thein vivo and DNA was isolated using the phenol–chloroform method. In brief, cells were lysed in a buffer containing 50mM Tris–HCl, pH 8.0, and 0.5% SDS, and incubated for 30min at 37 °C. The cell lysate was subjected to 8M potassium acetate precipitation and left for 1h at 4 °C. The supernatant was subjected to phenol/chloroform/isoamyl alcohol (25:24:1) extraction and once to chloroform extraction. DNA was precipitated by adding 1:2 volumes of ice-cold ethanol. The precipitated DNA was dissolved in 50ÃŽ ¼L TE buffer (pH 8.0). The DNA was digested with 20ÃŽ ¼g/mL RNase at 37 °C for 1h. The DNA was quantitated and equal concentration of DNA (25ÃŽ ¼g) was resolved on 1.5% agarose gel, viewed under UV light, and documented using BIORAD gel documentation system Figure 2 [259(b)]. Figure 2. The DNA degradation of compounds 4(a-e) in Ehrlich Ascites Tumor (EAT) cells. Conclusion: A series of 3-(4-(6,7-dihydro-5H-pyrrolo[1,2-c]imidazol-5-yl)-3-fluorophenyl)-5-substituted-1,2,4-oxadiazoles 4(a-e) has been synthesized by using simple synthetic procedures and were screened for their MTT assay using MCF-7 breast cancer cell line and degradation of DNA in Ehrlich Ascites Tumor (EAT) cells activity. All the final compounds exhibited good in all the in-vitro activity.

Ipswich Shellfish Delivers Fresh Lobster :: essays research papers

Ipswich Shellfish Company has a very unique marketing channel and strategy. There business is very specialized and their product characteristics will vary by fish, but not by quality. Ipswich is very confident that they can deliver fresh seafood within 24 hours around the world. Another product characteristic that they offer is a extensive variety of exotic seafood that people across the world can now enjoy. Some channel conflicts that they might run into is that they will deliver to anyone for the right price. This is much easier said then done but Ipswich delivers. To do this they need to have good relationships with their shipping affiliates. If their product goes bad then they lose money. Another problem that they might try to avoid would be sending out substandard seafood. If they do and their buyer tries to back out of the sale, they again will lose money. They are also facing rival businesses that must make their prices competitive and quality one grade better then other’s. It sounds like to me that Ipswich Shellfish must run a very tight business to survive. They are taking risks at many points and are relying for those risks to make profit. Therefore I would have to say that at this time it will not hinder their business to stay away from integration. This definitely will reduce the possibilities of extreme growth within their company, but also will reduce the possibilities of extreme losses that Ipswich could take. This way they are able to keep themselves out of the restaurant business, and out of the fishing business where they would have to sink a lot of money into something that they are not accustomed to. Intermodal coordination is key for this company to be able to distribute in such vast areas in such a small time. If they cannot coordinate between air carriers, land carriers and sea carriers then there product will go to waste. But on the other hand once they acquire the talent to coordinate their distribution channels they are able to serve many more markets and increase the volume of their sales. Being proficient with their shipping partners will enable them to gain security of their product. Without these companies Ipswich could not deliver shellfish very far, they would die within 24 hours if not properly cared for. This cost is great for Ipswich but essential in their growth process.

Research Paper Helicobacter Pylori

Helicobacter pylori| Previously named Campylobacter pyloridis, is a Gram-negative, microaerophilic bacterium found in the stomach. | | Microbiology (B1325) Research Paper| Detailed Introduction Helicobacter pylori are a species of epsilon proteobacteria which colonizes the harsh environment of the human stomach. Its name refers to both its spiral shape (Helicobacter) and the area of the lower stomach which it habitually colonizes: the gateway (pylorus) between the stomach and small intestine (Meyers, 2007).This bacterium is thought to be present within up to 50% of the human population and has been linked to the development of a number of different medical conditions (Chalmers et al. 2004). This report will provide information about the discovery of H. pylori as well as its morphological characteristics, taxonomic information, biochemical/metabolic characteristics, chemotherapeutic methods of control/treatment/eradication, immunological responses, pathological information, and epidem iology information. Morphological Characteristics Helicobacter pylori are a spiral-shaped, Gram-negative rod approximately 0. x 3. 0 micrometers in size. It is catalase-positive organism which has 4-6 sheathed flagella attached to one pole which allow for motility. It lives in the human stomach and duodenum. H. pylori possess five major outer membrane protein (OMP) families. The largest family includes known and putative adhesions. The other four families include porins, iron transporters, flagellum-associated proteins and proteins of unknown function. Like other typical Gram-negative bacteria, the outer membrane of H. pylori consists of phospholipids and lipopolysaccharide (LPS).The O antigen of LPS may be fucosylated and mimic Lewis blood group antigens found on the gastric epithelium. The outer membrane also contains cholesterol glucosides, which are found in few other bacteria. H. pylori has four to six lophotrichous flagella; all gastric and enterohepatic Helicobacter species a re highly motile due to flagella. The characteristic sheathed flagella filaments of Helicobacter are composed of two copolymerized flagellants, FlaA and FlaB. [1](From Wikipedia, the free encyclopedia). Taxonomic Information Helicobacter pylori are a gram-negative, spiral-shaped organism associated with gastrointestinal disease in humans.It has a worldwide prevalence, with approximately 50% of the world's population infected. Before the first isolation and documentation of this organism from the human stomach in 1982, it was assumed that the human stomach was a sterile environment because of the high levels of acid, which would exclude it as an ecologic niche for any organism. This bacterium is the human-adapted Helicobacter primarily found in the gastric mucosa and areas of gastric metaplasia in the duodenum and occasionally in Meckel's diverticulum and the rectum. It has been cultured rarely from feces, blood, and saliva.It can be detected by polymerase chain reaction (PCR) in den tal plaque and feces. In the latter instances, the viability of the bacteria is in question. H. pylori also have been found in nonhuman primates and cats. H. pylori detection in animals is not common and could be due to human contact with animals. To date, no environmental reservoir has been shown. [2](http://www. gastro. theclinics. com/article/S0889-8553(05)70135-7/abstract) Biochemical/Metabolic Characteristics The genus Helicobacter was created in 1989 with H. pylori as the type species. Since then the genus has expanded to include about 18 species.Some species were reclassified from Campylobacter, but most were newly discovered microorganisms from gastric or intestinal sites in mammalian host animals. The essential property of almost all helicobacter is the presence of sheathed flagella. Most species possess strong ureolytic ability, particularly those associated with gastric mucosa, and exhibit considerable diversity in cell morphology with respect to cell length, number and l ocation of flagella, and presence of periplasmic fibrils. H. pylori have a global distribution and infect human gastric mucosa exclusively but there is some evidence for infection in cats.Genomes of isolates from different individuals are unusual in their diversity in gene order and sequences within individual genes. ‘H. heilmannii1 is another gastric spiral shaped organism less frequently infecting humans but commonly found in cat and dog gastric tissue. H. felis is important in the mouse model of infection. A range of conventional phenotypic tests as well as some new PCR based assays are available for identifying isolates of Helicobacter from clinical specimens. [11]( http://bmb. oxfordjournals. org/content/54/1/17. full. pdf) Chemotherapeutic Methods of Control, Treatment, and EradicationTreatment If you are found to have Helicobacter pylori infection, you may wish to have antibiotic treatment of some kind. Treatment of Helicobacter pylori is usually simple ; straight forwa rd. However, occasional patients need repeated endoscopies, biopsies, breath tests and several courses of treatment with different antibiotic combinations. After treatment of H. pylori, it is necessary to repeat one of these tests to see if the germ has been killed or eradicated for good. Only breath tests or endoscopy with biopsy can be used to prove that the bacterium has been eradicated.The blood tests *(serology) is not suitable to monitor H. pylori eradication because antibodies to H. pylori may remain positive for months or even years after successfully killing the H. pylori. [3] (http://www. helico. com/? q=TreatmentForHelicobacterPylori) Eradication Prolonging the treatment period is a possible strategy for improving H. pylori eradication rates. Several studies have been published that tested this approach, including this paper by Calvet et al. These authors studied the value of extending PPI-based triple therapy from 7 to 10 days and found no additional benefit for patients with peptic ulcers.There was, however, a significant benefit for nonulcer dyspepsia patients (an increase from 66% to 77% in the intention-to-treat analysis and from 73% to 91% in the per-protocol analysis). The authors concluded that the treatment period should be extended from 7 to 10 days for patients with nonulcer dyspepsia. As most eradication therapy, however, is given to patients with uninvestigated dyspepsia, it is not unreasonable to argue that longer therapy should be given to all subjects. Distinguishing between patients with ulcer and nonulcer dyspepsia is therefore rather academic and impractical.The most obvious one is that existing PPI-based triple therapy regimens are not perfect. In the community at large, up to 30% of patients might fail this therapy. If clinicians prescribe triple therapy it should therefore be prescribed for longer than 7 days. This runs the risk of decreased patient compliance, more side effects and a greater cost, but ultimately it boils down to local and national guidelines, which vary from one country to another. Alternatively, clinicians might consider some of the newer eradication approaches, such as use of fluoroquinolone-based therapy or sequential treatment.The latter comprises quadruple therapy over a 10-day period, starting with a PPI plus amoxicillin (1,000 mg twice daily) for the first 5 days, followed by PPI plus clarithromycin (500 mg twice daily) and tinidazole (500 mg twice daily) for another 5 days. Intention-to-treat analysis eradication rates of 97%, 92%, and 94% have been reported in children, adults and elderly patients, respectively. Ultimately, clinicians should still strive towards a much simpler eradication strategy, but this will require investment in novel antibiotic discovery or a better understanding of the pathogenesis of H. ylori. Either way, there is much to be gained from continued interest in this little organism. [4](http://www. medscape. com/viewarticle/525100_2) Immunological Responses Lifelong Helicobacter pylori infection and its associated gastric inflammation underlie peptic ulceration and gastric carcinogenesis. The immune and inflammatory responses to H. pylori are doubly responsible: gastric inflammation is the main mediator of pathology, and the immune and inflammatory response is ineffective, allowing lifelong bacterial persistence.However, despite inducing gastric inflammation, most infections do not cause disease, and bacterial, host and environmental factors determine individual disease risk. Although H. pylori avoid many innate immune receptors, specific virulence factors (including those encoded on the cag pathogenicity island) stimulate innate immunity to increase gastric inflammation and increase disease risk. An acquired T helper 1 response up regulates local immune effectors. The extent to which environmental factors (including parasite infection), host factors and H. ylori itself influence T-helper differentiation and regulatory T-cell response s remains controversial. Finally, effective vaccines have still not been developed: a better understanding of the immune response to H. pylori may help. [5](http://www. ncbi. nlm. nih. gov/pubmed/17382275) Pathological Information Until the discovery of Helicobacter in 1982, ulcers were thought to be caused by stress. Now it is known that ulcers, in addition to gastritis, are caused by a bacterial infection of H. pylori. Though relatively easy to treat with antibiotics, H. ylori can be a risk factor for gastric cancer if it becomes a long-term infection [6] (Stated by D. J. Kelly, 2004. The University of Sheffield). The body's natural defenses cannot combat H. pylori because white and killer T cells cannot easily get through the stomach lining. The defense cells eventually die, spilling their superoxide radicals on stomach lining cells, on which H. pylori can feed[6] (Stated by Helicobacter Foundation, 2004). Epidemiology Information The frequency of H pylori infection in the United States may be linked to race. White persons account for 29% of cases, and Hispanic persons account for 60% of cases.Internationally, H pylori are a ubiquitous organism. At least 50% of all people are infected, but an exact determination is not available, mostly because exact data are not available from developing countries. H pylori may be detected in approximately 90% of individuals with peptic ulcer disease; however, less than 15% of infected persons may have this disease. The mortality rate related to H pylori infection is not precisely known, but it seems to be minimal (i. e. , approximately 2-4% of all infected people). Mortality is due to the complications of the infection, uch as gastric ulcer perforation or MALTomas of the GI tract. Otherwise, the morbidity of H pylori infection can be very high. [7](http://emedicine. medscape. com/article/176938-overview#a0199) The pathogenetic role of H pylori may differ depending on geography and race. White persons are infected with H p ylori less frequently than persons of other racial groups. The prevalence rate is approximately 20% in white persons, 54% in African American persons, and 60% in Hispanic persons. No sex predilection is known; however, females have a higher incidence of reinfection (5-8%) than males.H pylori infection may be acquired at any age. According to some epidemiologic studies, this infection is acquired most frequently during childhood. Children and females have a higher incidence of reinfection (5-8%) than adult males. [7](http://emedicine. medscape. com/article/176938-overview#a0199) Cultural Characteristics Approximately two-thirds of the world’s population is infected with H. pylori. In the United States, H. pylori are more prevalent among older adults, African Americans, Hispanics, and lower socioeconomic groups. It is not known how H. ylori are transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person thro ugh fecal-oral or oral-oral routes. Possible environmental reservoirs include contaminated water sources. Iatrogenic spread through contaminated endoscopes has been documented but can be prevented by proper cleaning of equipment. [8](Centers for disease and control prevention) Case Study 1 Title: Correlation of Helicobacter pylori and gastric carcinoma. Authors: Khanna, AK, Seth, P, Nath, G, Dixit, V K, Kumar, M Issue Date: 26-Jan-2002Citation: Khanna AK, Seth P, Nath G, Dixit VK, Kumar M. Correlation of Helicobacter pylori and gastric carcinoma. Journal of Postgraduate Medicine. 2002 Jan-Mar; 48(1): 27-8 Language: Eng. Type: Journal Article Abstract: BACKGROUND: Difference of opinion about the prevalence of H. pylori association with gastric cancer exists in the literature. AIMS: To study the correlation of Helicobacter pylori (H. pylori) to gastric carcinoma. METHODS: 50 proved cases of gastric cancer were studied by rapid urease test, culture, histopathology and ELISA test for H. pylori IgG.RESULTS: 68% of cases of gastric cancer were found to be positive for H. pylori infection as compared to 74% of healthy controls. CONCLUSIONS: The prevalence rate of H. pylori infection in our patients of gastric cancer was lower than in the control population though statistically not significant, suggesting that H. pylori may not be responsible for gastric carcinogenesis in this population. Source URI: http://www. jpgmonline. com URI: http://imsear. hellis. org/handle/123456789/116058 MeSH: * Adult * Case-Control Studies * Enzyme-Linked Immunosorbent Assay * Female Helicobacter Infections –complications * Helicobacter pylori –isolation & purification * Humans * Male * Middle Aged * Prevalence [9](http://imsear. hellis. org/handle/123456789/116058Stomach Neoplasms –microbiology) Case Study 2 Title: Helicobacter pylori in dental plaque of children and their family members. Authors: Gill, H H, Shankaran, K, Desai, H G Issue Date: 1-Sep-1994 Citation: G ill HH, Shankaran K, Desai HG. Helicobacter pylori in dental plaque of children and their family members. Journal of the Association of Physicians of India. 1994 Sep; 42(9): 719, 721 Language: Eng.Type: Journal Article Abstract: A prospective study was undertaken to determine the presence of Helicobacter pylori in the dental plaque of children and their family members. 22 children (age range: 2-12 years; males: 16) admitted to the pediatric ward for various disorders and 17 healthy family members (age range: 7-40 years; males: 9) of 13 of these children were screened for presence of Helicobacter pylori in the dental plaque by the rapid urease test. H. pylori were detected in dental plaque of 82% (18/22) children and 88% (15/17) of family members.In 85% (28/33) of the positive cases the rapid urease test was positive within 1 hour. Our observations indicate that Helicobacter pylori are present in the dental plaque of majority of children and their family members. Source URI: http://w ww. japi. org URI: http://imsear. hellis. org/handle/123456789/95238 MeSH: * Child * Child, Preschool * Dental Plaque –microbiology * Family Health * Female * Helicobacter pylori –isolation & purification * Humans * Male * Prospective Studies Appears in Collections: Journal of the Association of Physicians of India [10](http://imsear. ellis. org/handle/123456789/95238) Conclusion The author covered morphological characteristics, taxonomic information, biochemical/metabolic characteristics, chemotherapeutic methods of control/treatment/eradication, immunological responses, pathological information, and epidemiology in this paper. The overwhelming conclusion is that it is critical to survival of the human race that hygiene and education will be the best possible steps to overcome an increasing body of bacteria in our world. References 1. From Wikipedia, the free encyclopedia 2. http://www. gastro. theclinics. om/article/S0889-8553(05)70135-7/abstract) 3. http://www. heli co. com/? q=TreatmentForHelicobacterPylori 4. http://www. medscape. com/viewarticle/525100_2 5. http://www. ncbi. nlm. nih. gov/pubmed/17382275 6. Stated by D. J. Kelly, 2004. The University of Sheffield 7. http://emedicine. medscape. com/article/176938-overview#a0199 8. Centers for disease and control prevention 9. http://imsear. hellis. org/handle/123456789/116058Stomach Neoplasms –microbiology 10. http://imsear. hellis. org/handle/123456789/95238 11. http://bmb. oxfordjournals. org/content/54/1/17. full. pdf